Are you guys doing 16s or shotgun? Technical bias could potentially be introduced just by having different library prep methods (ligation vs tagmentation have their own gc biases) or even different collection methods (swabs, saliva, etc). Do you guys observe that? Are you guys classifying to known bacteria or are you also going to try to look at unknown things?
daemonk10 karma
Are you guys doing 16s or shotgun? Technical bias could potentially be introduced just by having different library prep methods (ligation vs tagmentation have their own gc biases) or even different collection methods (swabs, saliva, etc). Do you guys observe that? Are you guys classifying to known bacteria or are you also going to try to look at unknown things?
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