Tornpie

I am all about inflammation! I started studying all kinds of biomedical and bioengineering texts and research sometime after getting dismissed from graduate mathematics studies due to a combination of ADHD, chronic major depression, obstructive sleep apnea, and probably more. Through my studies and travels I started to notice people had problems that seemed to cluster around certain issues. Inflammation is a big one.

Since the biomedical and bioengineering stuff is huge in Pittsburgh because of UPMC and CMU, I have met several researchers and doctors. I've worked as a bouncer at a bar near the universities and hospitals and often drank with a transplant resident. I'd tell him my ideas, especially about inflammation and he found himself nodding you're right you're right. We often saw each other and would get excited to tell the other about any new thing we learned about cytokines.

I'll be honest with you. If I would have went to all the trouble you did, I would have made sure I had my own HPLC setup. Something I would like to do if I ever have the time and money is try to McGyver an HPLC from nonworking items and parts on ebay and wherever else I can find that stuff. I suspect a significant or even a dominant amount of immunomodulation takes place in concert with microbiome. The wikipedia only cites one paper with microbiome, inflammation and cancer but I'd imagine all kinds of stuff would show up on pubmed.

Inflammation could have very well been affected. Methylation and microbiome seems a bit beyond me at my current level of knowledge other than possible issues in how the gut absorbs folic acid, b6, b12, choline, and other methyl donors. It might be incredibly complicated in the end.

Not that I'm aware of. As far as the 23andme stuff, I've found digging through the raw data and fooling around on Google Scholar or SNPedia can give some insight. It will cross reference SNPs with papers. There are other third party tools. I agree with you somewhat on your views of the studies but it can't be totally bad and may give some leads. I think the big thing with microbiome problems are mediated through inflammation factors. As far as your other issues, methylation seems to be a factor particularly in bipolar. The methylation gene studies seems somewhat legit. I can dig up my methylation results to give you the main SNP numbers if you'd like.

Or you can use geneticgenie.org with raw data from 23andme. It's free and one of the tools I use.

I find this fascinating. My personal big four covering many ailments are inflammation (and immunomodulation), methylation, mitochondrial function, and endocrine balance which may be part of the cause of some of your problems. I believe that microbiome will eventually displace one of those. My question is have you had your 23andme done or some other gene testing done (perhaps you DIY'd that too) and did you do any before and after levels of inflammation factors, homocysteine, hormones, etc.?